Zoom in to at least 50% to see what the chromatograms look like in good vs poor quality regions. If you scroll down the sequences you’ll see that the sequence quality decreases dramatically at the end of each sequence. When zoomed out you won’t see the individual bases or chromatogram peaks, but there will be a graph visible giving an indication of sequence quality. This will highlight the base calls according to the quality of the sequence at that base – the darker the blue, the lower the quality. In the General tab to the right of the sequence view, choose to display Colors according to Quality. Double-click on the list to open it in a new window. Select the sequence list containing the raw sequence data from the mitochondrial DNA control region. The table below gives sampling location and codes for the sequences in this tutorial CodeĮxercise 1: Editing Mitochondrial DNA sequences
A sequence from the great tit Parus major is also included, as this would be a suitable outgroup for phylogenetic analysis. The dataset provided here comprises 34 sequences from the mitochondrial DNA control region of C. Mitochondrial DNA data can be used to investigate the phylogeography and population structure of these species. cyanus, found in Asia and eastern Europe. teneriffae, found in North Africa and the Canary Islands, and C. Mitochondrial DNA sequences – Introduction
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